Environmental Engineering
C. Tudsanaton; T. Pattamapitoon; O. Phewnil; W. Wararam; K. Chunkao; P. Maskulrath; M. Srichomphu
Abstract
BACKGROUND AND OBJECTIVES: Community domestic wastewater contains organic substances that can be decomposed through natural processes. Treatment using oxidation pond systems is popular in tropical zones because these locations provide a climate suitable for the growth and organic decomposition activities ...
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BACKGROUND AND OBJECTIVES: Community domestic wastewater contains organic substances that can be decomposed through natural processes. Treatment using oxidation pond systems is popular in tropical zones because these locations provide a climate suitable for the growth and organic decomposition activities of various bacteria that remove organic contaminants from wastewater. Given that bacteria play an important role in the biodegradation of organic substances in wastewater treatment plants, their degradation activity is used as an indicator of water quality. The purpose of this study is to examine the vertical variability of bacteria in natural treatment oxidation ponds in tropical zones.METHODS: Wastewater samples were collected from an oxidation pond at 3 different depths (0–0.6, 0.6–1.5 and more than 1.5 meter), and their chemical, physical and biological qualities were analysed. Next-generation sequencing techniques were used to identify bacterial diversity, and the water quality at each depth was applied as an indicator of bacterial degradation activities.FINDINGS: Community domestic wastewater contained 10 major bacterial phyla that differed at different depths. Amongst these phyla, the Actinobacteriota dominated (25.35 to 28.23 percent), followed by Cyanobacteria (19.49 to 21.57percent), Planctomycetota (15.50 to 17.41 percent), Firmicutes (9.97 to 10.79 percent), Proteobacteria (9.73 to 10.79 percent), Verrucomicrobiota (6.47 to 7.69 percent), Chloroflexi (2.79 to 2.99 percent), Bacteriota (0.96 to 1.41 percent), Acidobacteriota (0.70 to 0.80 percent) and SAR324 clade (marine group B) (0.69 to 0.61 percent). Four organic substances were found in contaminated domestic wastewater. 1) Photosynthetic cyanobacteria and phytoplankton performed aerobic degradation and accounted for the dissolved oxygen levels of 7.76, 7.45 and 7.42 milligrams per liter, respectively at various depths along the vertical profile. 2) Bacteria and archaeans that participate in carbon compound treatment included Planctomycetes, Verrucomicrobiota, Bacteroidota and Euryarcheota. These bacteria exhibited a treatment efficiency for biochemical oxygen demand and low abundance at all depths. Biological oxygen demand increased to 23.11, 24.27 and 34.48 milligrams per liter with depth. 3) Nitrogen-cycling bacteria included nitrifying and denitrifying bacteria. They belonged to the Actinobacteriota, Planctomycetota, Firmicutes, Verrucomicrobiota, Chloroflexi, Bacteriota, Protrobacteria and Acidobacteriota phyla. They exhibited an ammonia-nitrogen treatment efficiency of 91.73 percent. 4) Phosphorus cycling–related bacteria in the Actinobacteriota phylum presented the orthophosphorus treatment efficiency of 65.41 percent.CONCLUSION: The results of this study suggested that bacterial communities did not significantly differ along oxidation depth because they work together with chemotrophs, which participate in organic substance decomposition, and phototrophs, which are involved in oxygen generation and nutrient removal. All of the bacteria found in domestic wastewater benefit and support oxidation pond systems in tropical zones. Anaerobic bacteria can be found in wastewater and used in treatment systems due to their protective mechanisms against oxygen toxicity and self-repair mechanisms. The knowledge gained from this study can be used as a reference in future works on natural wastewater treatment systems.
Environmental Science
M. Srichomphu; O. Phewnil; T. Pattamapitoon; Ratcha Chaichana; K. Chunkao; W. Wararam; N. Dampin; P. Maskulrath
Abstract
BACKGROUND AND OBJECTIVES: As a producer within the ecological food chain, phytoplankton provides the base energy and oxygen to the environment through photosynthesis and higher tropic levels. These benefits can be applied in five consecutive nature-by-nature oxidation ponds for the treatment of community ...
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BACKGROUND AND OBJECTIVES: As a producer within the ecological food chain, phytoplankton provides the base energy and oxygen to the environment through photosynthesis and higher tropic levels. These benefits can be applied in five consecutive nature-by-nature oxidation ponds for the treatment of community wastewater coming through a high density polyethylene pipeline from the Phetchaburi Municipality located at the King’s Royally Initiated Laem Phak Bia Environmental Research and Development Project, Ban Laem District, Phetchaburi Province (Universal Transverse Mercator 47P 1442725 North 617774 East). This study focuses on the vertical distribution of the phytoplankton Cylindrospermopsis sp. and its relationship with nitrogen compounds in oxidation ponds.METHODS: Samples were collected from a community wastewater treatment system at various depths (30, 60, 90, 120, and 150 centimeters) below the water surface in April 2019 between 11:00 and 13:00 hours and analyzed for their chemical and physical properties. The analysis revealed a vertical relationship between Cylindrospermopsis sp. and wastewater. In the density of phytoplankton which were collected by measuring 20 liters of water and filtered using a 36-micron plankton net, calculated and counted under a high magnification microscope, as the species are classified according the taxonomy.FINDINGS: The results of the wastewater quality were as follows: the content of suspended solids was 65–81 milligram per liter, water temperature was 31.8–33.2°C, potential of hydrogen was 8.7–9.2, total nitrogen content was 4.0–5.3 milligram per liter, ammonium content was 0.03–0.06 milligram per liter, nitrate content was 0.09–0.12 milligram per liter, total phosphorus content was 0.9–1.3 milligram per liter, and phosphate content was 0.4–0.5 milligram per liter. In the density of phytoplankton, a significant correlation was observed between the population of Cylindrospermopsis sp. and water depth (R2 = 0.9324). The number of populations at the depths of 30, 60, 90, 120, and 150 centimeters were 3.2 × 107, 1.6 × 107, 1.1 × 107, 5.5 × 107, and 1.1 × 108 cells per cubic meter, respectively.CONCLUSION: The different densities of Cylindrospermopsis sp. found at different depths throughout the treatment pond are related to the nitrogen dynamics of the water body. The results of this study revealed that organic nitrogen, including ammonium, was assimilated and converted to inorganic nutrients, which promoted the growth of other phytoplankton species. The correlation between Cylindrospermopsis sp. and total nitrogen and ammonium showed significance at R2 = 0.7268 and 0.797, respectively, with a confidence level of 0.05. Therefore, to ensure treatment effectiveness, the depth of wastewater treatment ponds should be considered during their construction because phytoplankton regulation plays an important role to maintain the overall treatment efficiency.
Environmental Science
S. Saneha; T. Pattamapitoon; S. Bualert; O. Phewnil; W. Wararam; N. Semvimol; K. Chunkao; C. Tudsanaton; M. Srichomphu; U. Nachaiboon; O. Wongsrikaew; P. Wichittrakarn; C. Chanthasoon
Abstract
BACKGROUND AND OBJECTIVES: The bacterial community plays a crucial role in the nitrogen cycle. Oxidation ponds act as a natural treatment system for wastewater and are designed to promote the growth and activity of certain bacterial species that remove contaminants from the water. The nitrogen cycle ...
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BACKGROUND AND OBJECTIVES: The bacterial community plays a crucial role in the nitrogen cycle. Oxidation ponds act as a natural treatment system for wastewater and are designed to promote the growth and activity of certain bacterial species that remove contaminants from the water. The nitrogen cycle in these ponds involves the conversion of nitrogen compounds through biological processes by bacteria. The presence or absence of certain bacterial species can greatly influence the efficiency of the nitrogen cycle in these ponds. This research investigates the relationship between bacteria and nitrogen dynamics, the key components of wastewater treatment, in oxidation ponds. This work aims to identify the bacterial community composition in oxidation ponds, investigate the role of bacteria in the transformation and removal of nitrogen compounds from wastewater in oxidation ponds, and evaluate the impact of environmental factors on the microbial communities and nitrogen dynamics in oxidation ponds. This study was carried out in the oxidation wastewater treatment at the King’s Royally Initiated Laem Phak Bia Environmental Research and Development or LERD Project, in Phetchaburi, Thailand.METHODS: Wastewater samples were collected from the 1st–5th oxidation ponds at a depth of 30 centimeter from the water surface and analyzed for various quality parameters including temperature, dissolved oxygen, potential of hydrogen, biochemical oxygen demand, nitrates, ammonia, and total kjeldahl nitrogen. Next-generation sequencing by Illumina Miseq was used to examine the 16S ribosomal ribonucleic acid of bacteria in the collected samples. Correlation test was used for statistical analysis.FINDINGS: The temperature, potential of hydrogen (1st to 5th ponds), and dissolved oxygen (2nd to 5th ponds) in the oxidation ponds were within the standard value. Fifteen bacterial phyla were identified in the five oxidation ponds, with phylum Proteobacteria accounting for the highest population comprising 47.56% of the total bacterial population.CONCLUSION: Genera Novosphingobium (phylum Proteobacteria), Ammonia-11 (phylum Verrucomicrobiota), and Vicinamibacteraceae (phylum Acidobacteriota) have the strongest relationships with ammonia, nitrate, and total kjeldahl nitrogen (R2 = 0.9710, 0.986, 0.8124). The bacterial population is a crucial factor in nitrogen nutrient and water quality. Novosphingobium is involved in the removal of ammoniafrom wastewater, Verrucomicrobiota act as denitrifiers, and Vicinamibacteraceae increases the total kjeldahl nitrogen levels.
